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fluorescein labeled phaseolus vulgaris erythroagglutinin (pha-e)  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein labeled phaseolus vulgaris erythroagglutinin (pha-e)
    Fluorescein Labeled Phaseolus Vulgaris Erythroagglutinin (Pha E), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fl-1121/Fluorescein+labeled+Phaseolus+Vulgaris+Erythroagglutinin+(PHA-E)/custom%40fl-1121%4040769148
    Average 94 stars, based on 28 article reviews
    fluorescein labeled phaseolus vulgaris erythroagglutinin (pha-e) - by Bioz Stars, 2026-11
    94/100 stars

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    other:

    Article Title: Kidney-targeted antioxidant salvianolic acid B nanoparticles restoring lysosome homeostasis for acute kidney injury therapy
    Article Snippet: Acute kidney injury (AKI) is highly correlated with oxidative stress, and the application of antioxidants is one of the promising treatments.. Salvianolic acid B (SAB) is a natural polyphenol with powerful antioxidant effects.. Due to its inherent characteristic of inferior bioavailability, its clinical application is impeded.

    Saline:

    Article Title: O-GalNAc glycans are enriched in neuronal tracts and regulate nodes of Ranvier.
    Article Snippet: .. Following deparaffination with a xylene/ethanol gradient (which also removes lipids) and antigen retrieval, slides were washed with a solution of tris buffered saline with 0.1% Tween 20 (TBS- T), blocked with TBS- T + 3% bovine serum albumin (BSA) for 1 h at room temperature (RT), incubated with 25 μg/mL of lectins (Vector Labs: ConA FL- 1001, GNL FL- 1241, AAL FL- 1391, PHA- E FL- 1121, RCA- I FL- 1081, PNA FL- 1071, VVA FL- 1231) or anti- NeuN (α- NeuN) (Abcam, ab177487, 0.876 mg/mL), anti- GFAP (α- GFAP) (Sigma, HPA056030, 0.05 mg/mL), anti- Caspr (α- Caspr) (Abcam, Ab34151, 1 mg/mL), anti- MBP (α- MBP) AF594conjugated (Biolegend, 850905, 0.5 mg/mL) diluted at 1/100 in TBS- T or with MAG (Bio- Techne, 8580- MG, 200 μg/mL) diluted at 1/10 in TBS- T for 1 h at RT in darkness with gentle shaking. ..

    Incubation:

    Article Title: O-GalNAc glycans are enriched in neuronal tracts and regulate nodes of Ranvier.
    Article Snippet: .. Following deparaffination with a xylene/ethanol gradient (which also removes lipids) and antigen retrieval, slides were washed with a solution of tris buffered saline with 0.1% Tween 20 (TBS- T), blocked with TBS- T + 3% bovine serum albumin (BSA) for 1 h at room temperature (RT), incubated with 25 μg/mL of lectins (Vector Labs: ConA FL- 1001, GNL FL- 1241, AAL FL- 1391, PHA- E FL- 1121, RCA- I FL- 1081, PNA FL- 1071, VVA FL- 1231) or anti- NeuN (α- NeuN) (Abcam, ab177487, 0.876 mg/mL), anti- GFAP (α- GFAP) (Sigma, HPA056030, 0.05 mg/mL), anti- Caspr (α- Caspr) (Abcam, Ab34151, 1 mg/mL), anti- MBP (α- MBP) AF594conjugated (Biolegend, 850905, 0.5 mg/mL) diluted at 1/100 in TBS- T or with MAG (Bio- Techne, 8580- MG, 200 μg/mL) diluted at 1/10 in TBS- T for 1 h at RT in darkness with gentle shaking. ..

    Article Title: SLC35A2-mediated bisected GlcNAc-modified extracellular vesicles enhance immune regulation in breast cancer lung metastasis.
    Article Snippet: This study investigates the role of SLC35A2-mediated bisected GlcNAc-modified small extracellular vesicles (sEVs) in breast cancer (BC) lung metastasis.. By modulating B3GALT1 expression, these sEVs regulate the premetastatic immune microenvironment, enhancing CD8+ T cell infiltration and reducing immune evasion.. The use of β-peptide-loaded sEVs further amplifies anti-metastatic effects, as demonstrated in vivo mouse models and molecular analyses.

    Article Title: O-GalNAc glycans enrich in white matter tracts and regulate nodes of Ranvier
    Article Snippet: .. Tissues were washed again three times with TBS and incubated with 25 μg/mL of lectins (Vector Labs: ConA FL-1001, GNL B-1245, AAL FL-1391, PHA-E FL-1121, RCA-I B-1085, PNA FL-1071, VVA FL-1231) or a-NeuN (Abcam, ab177487, 0.876 mg/mL), a-GFAP (Sigma, HPA056030, 0.05 mg/mL), a-Caspr (Abcam, Ab34151, 1 mg/mL), a-MBP AF594-conjugated (Biolegend, 850905, 0.5 mg/mL) diluted at 1/100 in TBS-T or with MAG (Bio-Techne, 8580-MG, 200 μg/mL) diluted at 1/10 in TBS-T for 1 h at RT in darkness with gentle shaking. ..

    Gentle:

    Article Title: O-GalNAc glycans are enriched in neuronal tracts and regulate nodes of Ranvier.
    Article Snippet: .. Following deparaffination with a xylene/ethanol gradient (which also removes lipids) and antigen retrieval, slides were washed with a solution of tris buffered saline with 0.1% Tween 20 (TBS- T), blocked with TBS- T + 3% bovine serum albumin (BSA) for 1 h at room temperature (RT), incubated with 25 μg/mL of lectins (Vector Labs: ConA FL- 1001, GNL FL- 1241, AAL FL- 1391, PHA- E FL- 1121, RCA- I FL- 1081, PNA FL- 1071, VVA FL- 1231) or anti- NeuN (α- NeuN) (Abcam, ab177487, 0.876 mg/mL), anti- GFAP (α- GFAP) (Sigma, HPA056030, 0.05 mg/mL), anti- Caspr (α- Caspr) (Abcam, Ab34151, 1 mg/mL), anti- MBP (α- MBP) AF594conjugated (Biolegend, 850905, 0.5 mg/mL) diluted at 1/100 in TBS- T or with MAG (Bio- Techne, 8580- MG, 200 μg/mL) diluted at 1/10 in TBS- T for 1 h at RT in darkness with gentle shaking. ..

    Article Title: O-GalNAc glycans enrich in white matter tracts and regulate nodes of Ranvier
    Article Snippet: .. Tissues were washed again three times with TBS and incubated with 25 μg/mL of lectins (Vector Labs: ConA FL-1001, GNL B-1245, AAL FL-1391, PHA-E FL-1121, RCA-I B-1085, PNA FL-1071, VVA FL-1231) or a-NeuN (Abcam, ab177487, 0.876 mg/mL), a-GFAP (Sigma, HPA056030, 0.05 mg/mL), a-Caspr (Abcam, Ab34151, 1 mg/mL), a-MBP AF594-conjugated (Biolegend, 850905, 0.5 mg/mL) diluted at 1/100 in TBS-T or with MAG (Bio-Techne, 8580-MG, 200 μg/mL) diluted at 1/10 in TBS-T for 1 h at RT in darkness with gentle shaking. ..

    Membrane:

    Article Title: SLC35A2-mediated bisected GlcNAc-modified extracellular vesicles enhance immune regulation in breast cancer lung metastasis.
    Article Snippet: This study investigates the role of SLC35A2-mediated bisected GlcNAc-modified small extracellular vesicles (sEVs) in breast cancer (BC) lung metastasis.. By modulating B3GALT1 expression, these sEVs regulate the premetastatic immune microenvironment, enhancing CD8+ T cell infiltration and reducing immune evasion.. The use of β-peptide-loaded sEVs further amplifies anti-metastatic effects, as demonstrated in vivo mouse models and molecular analyses.

    Blocking Assay:

    Article Title: SLC35A2-mediated bisected GlcNAc-modified extracellular vesicles enhance immune regulation in breast cancer lung metastasis.
    Article Snippet: This study investigates the role of SLC35A2-mediated bisected GlcNAc-modified small extracellular vesicles (sEVs) in breast cancer (BC) lung metastasis.. By modulating B3GALT1 expression, these sEVs regulate the premetastatic immune microenvironment, enhancing CD8+ T cell infiltration and reducing immune evasion.. The use of β-peptide-loaded sEVs further amplifies anti-metastatic effects, as demonstrated in vivo mouse models and molecular analyses.



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Functional genomics identifies N -acetyllactosamine extension of complex N -glycans as a mechanism to evade lysis by natural killer cells

    doi: 10.1016/j.celrep.2024.114105

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Phaseolus Vulgaris Erythroagglutinin (PHA-E), FITC , Vector labs , FL-1121-2.

    Techniques: Virus, Cell Isolation, Recombinant, Plasmid Preparation, Selection, CRISPR, Knock-Out, Software